Tetrahydrobiopterin Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Tetrahydrobiopterin in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a competitive inhibition Sandwich ELISA in strip well format with reagents for up to 96 tests . THB present in samples reacts with an anti-THB monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, THB conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active THB in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of THB. Detection Target: Tetrahydrobiopterin Reactivity: Multi-species Range: 0.156-10ng/mL Sensitivity: 0.056ng/mL Storage Conditions: Store all components at 4┬░C This Multi-species THB activity assay is for the quantitative determination of Tetrahydrobiopterin in samples including serum, plasma, tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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