Single Donor Human Buffy Coats LeukocytesIWB5ACDUNIT
Single Donor Human Buffy Coats / Leukocytes from Innovative Research are processed from a single donor whole blood collection (approximately 450ml starting volume) collected at FDA-licensed facilities located in the United States. The blood is collected into a sterile collection bag with the anticoagulant ACD (additional anticoagulant options available upon request).
Buffy coat preparations are separated from the whole blood using low-speed centrifugation. The buffy coat layer is manually extracted (no chemical separators are used) and is generally enriched for leukocytes (WBCs / white blood cells) and contain trace amounts of erythrocytes (RBCs / red blood cells) and other blood components. The resulting volume is approximately 50ml-60ml. If desired, you may further purify this preparation of leukocytes by sedimentation using Percoll gradient centrifugation.
This product is suitable as a source of nucleated PBMC, and is prepared in a buffer composed of 20.5 mg/ml Dextrose, 4.2mg/ml Sodium Chloride, 8.0 mg/ml Citric Acid Tri-sodium, adjusted to pH 6.1. The recommended storage temperature for this material is 2-8°C
Each unit is tested for the standard FDA-required viral markers, and found negative for HBsAg, HCV, HIV-1, HIV-2, HIV-1Ag or HIV-1 NAT, ALT, and syphilis using FDA-approved methods. If you would like any further testing or analysis performed prior to shipping, please let us know.
Innovative Research has a wide network of FDA-approved collection centers across the United States, which allows us to collect from nearly any donor type. We are able to accommodate demographic requests from a variety of genders, races, and ages. We are also able to work with custom collection requests and protocols. If you have custom demographic or collection requests, please let us know.
Please note - this product replaces our old product number IPLA-WB5 (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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