Rat Total Urokinase (uPA) ELISA Kit
Rat uPA total antigen assay is intended for the quantitative determination of total urokinase plasminogen activator in rat plasma, serum, urine, cell culture media, or tissue extracts. For research use only. Strip well format. Reagents for up to 96 tests.
Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
- Detection Target: uPA
- Reactivity: Rat
- Range: 0.025-10 ng/ml
- Sensitivity: 0.01 ng/ml
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Please note – this product replaces our old product number IRUPAKT-TOT (same product, new number).