Mouse tPA Genetically Deficient Heart Tissue Lysate

Mouse tPA Genetically Deficient Heart Tissue Lysate from Innovative Research is prepared from a tPA knockout mouse. This Tissue Plasminogen Activator (tPA) Knockout was created by targeted disruption of the PLAT gene. Mutations were then confirmed using PCR genotyping and Sanger sequencing. Organ is flash-frozen immediately upon removal and stored frozen until lysed. The tissue is then lyophilized and thorougly homogenized in Radio Immuno Precipitation Assay (RIPA) buffer containing protease and phosphate incubators. The final homogenate is then incubated with DNase, clarified via centrifugation (10,000g at 4░C) and stored at -80░C. Each lot is tested for protein concentration via BCA assay and analyzed via SDS-PAGE to confirm the presence of sharp, clear bands as an indicator of quality. Product Details: Species:Mouse Antigen:tPA Format:Tissue Lysate Storage Conditions:-70 C This product can be useful as a control in a wide range of in vitro applications, including ELISA and Western Blot, among others. Useful for research in cardiovascular and neurological studies. Mouse Model Details Strain:C57BL6/SJL Chromosome:8 Gene:PLAT Genomic TargetExon 4 Mutation:16 base pair deletion Phenotype:Complete absence of tPA expression as confirmed by Western Blot of brain tissue lysates, loss of tPA activity confirmed by ELISA. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.