Mouse Serum Amyloid P (SAP) ELISA Kit

The Mouse Serum Amyloid P (SAP) ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAP in Mouse biological samples. Strip well format. Reagents for up to 96 tests. In this assay the SAP present in samples reacts with the anti-SAP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-SAP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAP in the test sample. The quantity of SAP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. More Details: Detection Target: Serum Amyloid P Reactivity: Mouse Range: 6.25 ng/ml - 200 ng/ml Sensitivity: 1.845 ng/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ¢ this product replaces our old product number IRKTAH1163 (same product, new number).