Mouse Profilin Toxoplasma gondii Recombinant Protein Tag Free Lyophilized from Innovative Research has been recombinantly produced in E. coli. This is a Lyophilized protein buffered in Contains PBS. Reconstitute with 100 and#956;l sterile water. with a purity of >95% (SDS-PAGE).
Endotoxin level is less than 0.01EU/ and#956;g purified protein (LAL test; Lonza)..
More Details:
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Species: Mouse
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Target:
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Purity: >95% (SDS-PAGE).
Endotoxin level is less than 0.01EU/ and#956;g purified protein (LAL test; Lonza).
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Source: E. coli
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Storage Conditions: Stable for at least 6 months after receipt when stored at -20°C.
Additional Information:
TLR11, an innate sensor for profilin (Toxoplasma gondii), is an intracellular receptor that resides in the endoplasmic reticulum. The 12 membrane-spanning endoplasmic reticulum-resident protein UNC93B1 interacts directly with TLR11 and regulates the activation of dendritic cells in response to profilin (Toxoplasma gondii) and parasitic infection in vivo. TLR12, a previously uncharacterized TLR, also recognizes profilin from Toxoplasma gondii. TLR12 is sufficient for recognition of profilin by plasmacytoid dendritic cells (pDCs), whereas TLR11 and TLR12 are both required in macrophages and conventional DCs. In contrast to TLR11, TLR12-deficient mice succumb rapidly to T. gondii infection.
This recombinant proteins is for research use only.. At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results.
Curious About The Best Way To Thaw Plasma?
- Preheat a water bath to 37 C.
- Place the frozen plasma sample directly in the water bath.
- Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
- The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
- The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
- Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
- Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
- Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.
This protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results
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