Mouse IgE ELISA KitIMSIGEKT
The Mouse IgE ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in biological samples of mice. Strip well format. Reagents for up to 96 tests.
The assay is a double antibody sandwich Mouse IgE ELISA. In this assay the IgE present in samples reacts with the anti-Mouse IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
- Detection Target: IgE
- Reactivity: Mouse
- Range: 6.25 ng/ml - 400 ng/ml
- Sensitivity: 1.911 ng/ml
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Please note – this product replaces our old product number IMSIGEKT (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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