Mouse Factor XII Genetically Deficient Lung Tissue Lysate

Mouse Factor XII Genetically Deficient Lung Tissue Lysate from Innovative Research is prepared from a Factor XII knockout mouse. This Factor XII Knockout was created by targeted disruption of the F12 gene. Mutations were then confirmed using PCR genotyping and Sanger sequencing. Organ is flash-frozen immediately upon removal and stored frozen until lysed. The tissue is then lyophilized and thorougly homogenized in Radio Immuno Precipitation Assay (RIPA) buffer containing protease and phosphate incubators. The final homogenate is then incubated with DNase, clarified via centrifugation (10,000g at 4░C) and stored at -80░C. Each lot is tested for protein concentration via BCA assay and analyzed via SDS-PAGE to confirm the presence of sharp, clear bands as an indicator of quality. Product Details: Species:Mouse Antigen:Factor XII Format:Tissue Lysate Storage Conditions:-70 C This product can be useful as a control in a wide range of in vitro applications, including ELISA and Western Blot, among others. Useful for research in areas like cardiovascular and neurological studies. Mouse Model Details Strain:C57BL6/SJL Chromosome:13 Gene:F12 Genomic TargetExon 8-12 Mutation:Large deletions Phenotype:Complete absence of FXII in plasma as confirmed by Western Blot. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.