Mouse Anti Keyhole Limpet Hemocyanin (anti-KLH) IgG ELISA Kit

The Mouse Anti Keyhole Limpet Hemocyanin (anti-KLH) IgG ELISA Kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses KLH for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-mouse IgG antibodies for detection.. Strip well format. Reagents for up to 96 tests.

The mouse anti-KLH IgG test kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses KLH for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-mouse IgG antibodies for detection. Test serum or plasma samples are diluted and incubated in the microtiter wells for 1 hour. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. Anti-KLH IgG molecules are thus sandwiched between immobilized KLH and the detection antibody conjugate. The wells are then washed to remove unbound HRP- labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of anti-KLH IgG is proportional to the optical density of the test sample.

More Details:

• Detection Target: Anti-KLH IgG
• Reactivity: Mouse
• Range: 6.25-100 u/ml

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Please note – this product replaces our old product number IRKTAH3024 (same product, new number).