Mouse Active tPA ELISA KitIMSTPAKTA
This mouse tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator in mouse plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests.
Functionally active mouse tPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, monoclonal anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
- Detection Target: tPA
- Reactivity: Mouse
- Range: 0.05-50 ng/ml
- Sensitivity: 0.02 ng/ml
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Please note – this product replaces our old product number IMTPAKT (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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