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*Immunoglobulin G (IgG) Depleted Human Serum
*Immunoglobulin G (IgG) Depleted Human Serum
SKU:IIGGDS100ML
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Pooled human serum with IgG depleted via immobilized Protein A, prepared from human serum. Offered in 100 mL. Certificate of Analysis available on request. Formerly Innovative Research SKU IHPLA-SER-GF; also known as Molecular Innovations HPLA-SER-GF.
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In-depth information
Certificate of Analysis
Contact customer support to get your certificate of analysis
Select this product for Molecular Innovations name, Human IgG depleted serum and product number, HPLA-SER-GF.
Immunoglobulin G (IgG) Depleted Human Serum from Innovative Research is prepared from high-quality human serum using immobilized Protein A.
Please note: this product replaces our old product number IHPLA-SER-GF (same product, new number).
- Preheat a water bath to 37 C.
- Place the frozen plasma sample directly in the water bath.
- Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
- The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
- The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
- Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
- Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
- Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.
a - No known test method can offer complete assurance that infectious agents are absent. All material should be handled as potentially infectious. All samples should be handled following universal precautions as outlined by WHO, CDC, and OSHA — biological materials should always be treated as if capable of transmitting infectious disease
b - Each lot is tested for hemolytic complement activity via CH50 assay. Result reported on the Certificate of Analysis
| Specification | Detail |
|---|---|
| Product Name | Immunoglobulin G (IgG) Depleted Human Serum |
| SKU | IIGGDS100ML |
| Legacy SKU | IHPLA-SER-GF (replaced by current SKU) |
| Cross-reference | Molecular Innovations HPLA-SER-GF (same product) |
| Source | Pooled human serum |
| Processing | IgG depleted via immobilized Protein A |
| Volume | 100 mL |
| Viral testing | Same standard FDA panel as other human serum products - a |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. Not for human or animal use. |
| Storage Temperature | -20C |
| Shelf Life | 3 years from manufacture date |
| Shipping Conditions | Frozen, dry ice |
| Certificate of Analysis (CoA) | Available on request |
| Safety Data Sheet (SDS) | Available for download |
| Product Datasheet | Available for download |
| Country of origin | United States |
| Manufacturer | Innovative Research, Inc. (Novi, MI) |
| Lead Time | Usually ships within 1-2 weeks from order confirmation |
This material is sold for in-vitro use only for research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.
Publications
and citations
Frequently Asked Questions
Immunoglobulin G is removed from the serum through the use of immobilized Protein A, which selectively binds IgG and allows the remainder of the serum matrix to pass through. The result is a serum matrix with substantially reduced IgG content, suitable for research where endogenous IgG would interfere with the experimental readout. Residual IgG levels tested via ELISA and reported on individual Certificate of Analysis.
Yes. Innovative Research's Immunoglobulin G (IgG) Depleted Human Serum is also known by the Molecular Innovations name, Human IgG depleted serum, product number HPLA-SER-GF. It also replaces our previous SKU IHPLA-SER-GF — same product, new number.
This product is pooled. If you need a single-donor version, see our Single Donor Immunoglobulin G (IgG) Depleted Human Serum product, or contact our team about custom pooled lots to specific donor specifications.
The product ships in 100 mL. If you require a different volume or custom aliquots, contact our team before placing your order.
Yes. Human serum products from Innovative Research are tested using FDA-approved methods for HBsAg, HCV, HIV-1/HIV-2 Antigen Antibody Combo, and Anti-T. Pallidum, and found negative before shipment. All samples should be handled following universal precautions as outlined by WHO, CDC, and OSHA — biological materials should always be treated as if capable of transmitting infectious disease.
Immunoglobulin G (IgG) Depleted Human Serum is intended For Research Use Only. Not for use in diagnostic procedures. Not for human or animal use. Common applications include research immunoassay development, autoantibody research, complement pathway research, immunoprecipitation and Western blot method development, protein-protein interaction research, and comparison studies. See the Research Applications section above for a more complete list.
Yes. A Certificate of Analysis is available on request for every Innovative Research IgG depleted serum product. Contact customer support after placing your order, or reach out before ordering if your project requires CoA review prior to purchase.
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