Sheep Haptoglobin ELISA Kit
Sheep Haptoglobin ELISA Kit from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Sheep Haptoglobin in biofluid samples. Strip well format. Reagents for up to 96 tests.
In this assay the HPT present in samples reacts with the anti-HPT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HPT antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound HPT. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HPT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HPT in the test sample. The quantity of HPT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
- Detection Target: Haptoglobin
- Reactivity: Sheep
- Range: 15.625 ng/ml - 1000 ng/ml
- Sensitivity: 5.047 ng/ml
- Storage Conditions: 4C
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