Rat Visceral Adipose-Specific Serpin (Serpin A12) ELISA Kit

This Rat Visceral Adipose-Specific Serpin (Serpin A12) ELISA Kit from Innovative Research is intended for quantitative detection of rat Serpin A12 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This rat Serpin A12 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin A12 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L21-P411) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Serpin A12 amount of sample captured in plate.

• Detection Target: Visceral Adipose-Specific Serpin (Serpin A12)
• Uniprot ID: Q8R4Z1)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: ; Immunogen sequence: 369