Rat Uteroglobulin-Related Protein 2 ELISA Kit

This Rat Uteroglobulin-Related Protein 2 ELISA Kit from Innovative Research is intended for quantitative detection of rat SCGB3A1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. This rat SCGB3A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCGB3A1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: F22-G104) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB3A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate. Detection Target: Uteroglobulin-Related Protein 2 Uniprot ID: E9PU36) Reactivity: Rat Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins. Range: 15.6pg/ml-1000pg/ml Sensitivity: <10pg/ml Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice) Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. It is mapped to 10q21. Members of the secretoglobin family, such as SCGB3A1, are secreted proteins of about 10 kD that are found in high concentrations in fluids of lung, lacrimal gland, salivary gland, prostate, uterus, and other tissues. ; Immunogen sequence: 369