Rat Semaphorin 6C ELISA Kit

This Rat Semaphorin 6C ELISA Kit from Innovative Research is intended for quantitative detection of rat SEMA6C in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.

This rat SEMA6C ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SEMA6C has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A24-P635) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEMA6C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat SEMA6C amount of sample captured in plate.

• Detection Target: Semaphorin 6C
• Uniprot ID: Q9WTL3)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 2q34. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration.; Immunogen sequence: 369