Rat Myoglobin ELISA Kit from Innovative Research s a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Rat Myoglobin in biological samples. Strip well format. Reagents for up to 96 tests. In this assay the Myoglobin present in samples reacts with the antiMyoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, antiMyoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzymelabeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Myoglobin Reactivity: Rat Range: 6.25 ng/ml - 200 ng/ml Sensitivity: 3.612 ng/ml Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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