Rat Interleukin-6 Receptor Beta (GP130) ELISA KitIRTIL6STKT
This Rat Interleukin-6 Receptor Beta (GP130) ELISA Kit from Innovative Research is intended for quantitative detection of rat IL6ST in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This rat IL6ST ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL6ST has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q23-E618) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL6ST is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
- Detection Target: Interleukin-6 Receptor Beta (GP130)
- Uniprot ID: P40190)
- Reactivity: Rat
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 0.78ng/ml-50ng/ml
- Sensitivity: <0.05ng/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal. ; Immunogen sequence: 369
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