Rat Interleukin-18 (IL-18) ELISA Kit

This Rat Interleukin-18 (IL-18) ELISA Kit from Innovative Research is intended for quantitative detection of rat IL-18 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This rat IL-18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-18 has been precoated onto 96-well plates. Standards(E.coli, H37-S194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-18 amount of sample captured in plate.

• Detection Target: Interleukin-18 (IL-18)
• Uniprot ID: P97636)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <1pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a polyclonal antibody from goat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3; Immunogen sequence: 400