Rat Growth Differentiation Factor 15 ELISA Kit

Rat Growth Differentiation Factor 15 ELISA Kit

IRTGDF15KT

Innovative Research

  • $744.00


This Rat Growth Differentiation Factor 15 ELISA Kit from Innovative Research is intended for quantitative detection of rat GDF-15 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This rat GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat GDF-15 amount of sample captured in plate.

  • Detection Target: Growth Differentiation Factor 15
  • Uniprot ID: Q9Z0J6)
  • Reactivity: Rat
  • Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
  • Range: 15.6pg/ml-1000pg/ml
  • Sensitivity: <10pg/ml
  • Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: GDF-15(Growth differentiation factor 15), also known as TGF-PL, MIC-1, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.; Immunogen sequence: 369

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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