Rat Galectin-1 ELISA KitIRTLGALS1KT
This Rat Galectin-1 ELISA Kit from Innovative Research is intended for quantitative detection of rat Galectin-1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This rat Galectin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-1 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A2-E135? and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Galectin-1 amount of sample captured in plate.
- Detection Target: Galectin-1
- Uniprot ID: P11762)
- Reactivity: Rat
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 156pg/ml-10000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.; Immunogen sequence: 399
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