Rat FMS-Related Tyrosine Kinase 3 Ligand (Flt-3 Ligand) ELISA Kit

This Rat FMS-Related Tyrosine Kinase 3 Ligand (Flt-3 Ligand) ELISA Kit from Innovative Research is intended for quantitative detection of rat Flt-3 ligand in cell culture supernates and serum. Strip well format. Reagents for up to 96 tests.

This rat Flt-3 ligand ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Flt-3 ligand has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G27-R188) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3 ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Flt-3 ligand amount of sample captured in plate.

• Detection Target: FMS-Related Tyrosine Kinase 3 Ligand (Flt-3 Ligand)
• Uniprot ID: A0A1W2Q6J3)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.; Immunogen sequence: 369