Rat Erythropoietin ELISA Kit

This Rat Erythropoietin ELISA Kit from Innovative Research is intended for quantitative detection of rat EPO in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.

This rat EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPO has been precoated onto 96-well plates. Standards(E.coli, T44-K226) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat EPO amount of sample captured in plate.

• Detection Target: Erythropoietin
• Uniprot ID: P29676)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 46.9pg/ml-3000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What’s more, EPO is also involved in the wound healing process.; Immunogen sequence: 369