This Rat Cadherin-5 (VE-Cadherin) Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of rat VE-Cadherin/CD144 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This rat VE-Cadherin/CD144 Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VE-Cadherin/CD144 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: D46 - Q599) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VE-Cadherin/CD144 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
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Detection Target: Cadherin-5 (VE-Cadherin)
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Uniprot ID: F1M7E5)
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Reactivity: Rat
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 312pg/ml-20000pg/ml
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Sensitivity: <50pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Cadherin 5, type 2 or VE-cadherin also known as CD144, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 19p14. VE-cadherin is a classical cadherin from the cadherin superfamily and the gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.; Immunogen sequence: 333
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