Rat Bone Morphogenetic Protein 10 ELISA Kit

This Rat Bone Morphogenetic Protein 10 ELISA Kit from Innovative Research is intended for quantitative detection of rat BMP-10 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This rat BMP-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-10 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: N314-R421) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: Bone Morphogenetic Protein 10
• Uniprot ID: Q4AEG6)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-? superfamily of structurally related signaling proteins. This gene is mapped to 4q34. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life.  BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation.  It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.  BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.  Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor. ; Immunogen sequence: 333