Rabbit Active tPA ELISA KitIRBTPAKTA
Rabbit Active tPA ELISA Kit from Innovative Research is intended for the quantitative determination of active tissue plasminogen activator in rabbit plasma and other biological fluids. Strip well format. Reagents for up to 96 tests.
Functionally active rabbit tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active tPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm.
- Detection Target: Active tPA
- Reactivity: Rabbit
- Cross-Reactivity: Does not significantly cross react with human, rat, or Porcine.
- Range: 0.5?100 ng/ml
- Sensitivity: 0.181ng/ml
- Storage Conditions: 2-8°C
Please note, pooled normal plasma from sheep resulted in significant color development. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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