Porcine Metalloproteinase Inhibitor 3 High-Sensitivity ELISA Kit

This Porcine Metalloproteinase Inhibitor 3 High-Sensitivity ELISA Kit from Innovative Research is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of Pig porcine TIMP-3. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.

This Pig TIMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-3 has been precoated onto 96-well plates. Standards(NSO, C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Pig TIMP-3 amount of sample captured in plate.

• Detection Target: Metalloproteinase Inhibitor 3
• Uniprot ID: P35625)
• Reactivity: Porcine
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10,000pg/ml
• Sensitivity: <2pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).; Immunogen sequence: 400