This Porcine Endothelin 1 High-Sensitivity Sandwich ELISA Kit from Innovative Research is intended is a high-sensitivity sandwich Sandwich ELISA kit for quantitative detection of Pig porcine Endothelin. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.
This Pig Endothelin Sandwich ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Endothelin has been precoated onto 96-well plates. Standards(human peptide, C53-W73) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Pig Endothelin amount of sample captured in plate.
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Detection Target: Endothelin 1
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Uniprot ID: P05305)
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Reactivity: Porcine
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 3.9pg/ml-250pg/ml
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Sensitivity: <0.5pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of Pig porcine endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.; Immunogen sequence: 369
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