Mouse Tumor Necrosis Factor Receptor Superfamily Member 8 (CD30) ELISA Kit

This Mouse Tumor Necrosis Factor Receptor Superfamily Member 8 (CD30) ELISA Kit from Innovative Research is intended for quantitative detection of mouse CD30 in cell culture supernates and serum. Strip well format. Reagents for up to 96 tests.

This mouse CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-T281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30 amount of sample captured in plate.

• Detection Target: Tumor Necrosis Factor Receptor Superfamily Member 8 (CD30)
• Uniprot ID: Q60846)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.; Immunogen sequence: 369