This Mouse Tumor Necrosis Factor Receptor Superfamily Member 6 (FAS) ELISA Kit from Innovative Research is intended for quantitative detection of mouse FAS in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This mouse FAS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FAS has been precoated onto 96-well plates. Standards(sf21, Q22-R169) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FAS amount of sample captured in plate.
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Detection Target: Tumor Necrosis Factor Receptor Superfamily Member 6 (FAS)
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Uniprot ID: P25446)
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Reactivity: Mouse
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 31.2pg/ml-2000pg/ml
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Sensitivity: <3pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.; Immunogen sequence: 300
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