Mouse Tumor Necrosis Factor Receptor Superfamily Member 19 (TRADE) ELISA Kit

This Mouse Tumor Necrosis Factor Receptor Superfamily Member 19 (TRADE) ELISA Kit from Innovative Research is intended for quantitative detection of mouse TNFRSF19 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This mouse TNFRSF19 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF19 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E30-L170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF19 amount of sample captured in plate.

• Detection Target: Tumor Necrosis Factor Receptor Superfamily Member 19 (TRADE)
• Uniprot ID: Q9JLL3)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.; Immunogen sequence: 369