*Mouse Total Factor II (Prothrombin) ELISA Kit

Select this product for Molecular Innovations name, Mouse Prothrombin Total Antigen ELISA Kit and product number, MPTKT-TOT. Mouse Total Factor II (Prothrombin) ELISA Kit from Innovative Research is intended for the quantitative determination of total prothrombin in mouse plasma, serum, tissue extracts, and cell culture media. Thrombin and thrombin-antithrombin complex will be detected by the assay. We also offer a Mouse Factor II (Prothrombin) ELISA Kit that is specific for prothrombin and will not detect thrombin or TAT complex. For research use only. Strip well format. Reagents for up to 96 tests. Mouse prothrombin will bind to the capture antibody coated on the microtiter plate. Thrombin and thrombin-antithrombin complex will not react with the antibody coated on the plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color development is directly proportional to the concentration of prothrombin in the sample. More details: Detection Target: Total Factor II (Prothrombin)Reactivity: MouseCross-Reactivity: Significant cross-reaction is observed with rat. Does not significantly cross react with human, porcine, rabbit, or sheep.Range: 1-500 ng/mlSensitivity: 0.52 ng/mlStorage Conditions: 2-8░C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note this product replaces our old product number IMPTKT-TOT (same product, new number).