Mouse Thrombopoietin ELISA Kit

This Mouse Thrombopoietin ELISA Kit from Innovative Research is intended for quantitative detection of mouse TPO in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This mouse TPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TPO has been precoated onto 96-well plates. Standards(NSO, S22-T356) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TPO amount of sample captured in plate.

• Detection Target: Thrombopoietin
• Uniprot ID: P40226)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <5pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.; Immunogen sequence: 340