This Mouse T-Lymphocyte Surface Antigen Ly 9 Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of mouse Ly9 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This mouse Ly9/SLAMF3/CD229 Sandwich ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Ly9/SLAMF3/CD229 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: K48-R453) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ly9/SLAMF3/CD229 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Ly9/SLAMF3/CD229 amount of sample captured in plate.
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Detection Target: T-Lymphocyte Surface Antigen Ly 9
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Uniprot ID: Q01965)
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Reactivity: Mouse
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 93.7pg/ml-6000pg/ml
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Sensitivity: <50pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 1 H3; 1 79.54 cM. Mature human CD229 consists of a 407 amino acid (aa) extracellular domain (ECD) with two Ig?like V-set and two Ig?like truncated C2-set domains. It also shows a 22 aa transmembrane segment, and a 179 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs. The ECD of human CD229 shares 57%?59% aa sequence identity with mouse and rat CD229. Within the first two Ig?like domains that are common to all SLAM proteins, human CD229 shares 24%?39% aa sequence identity with human 2B4, BLAME, CD2F-10, CD84, CRACC, NTB-A, and SLAM. Alternate splicing generates two additional isoforms that lack the juxtamembrane Ig?like domain or short cytoplasmic region. CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between CD229 molecules is mediated by the N-terminal Ig?like domain. Human and mouse CD229 exhibit cross-species binding. Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell - B cell contact. Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (?2 chain) and GRB-2 and both are required for CD229 internalization.; Immunogen sequence: 369
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