This Mouse Stromal Cell-Derived Factor 1 (CXCL12) Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of mouse SDF-1 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.
This mouse SDF-1 Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SDF-1 has been precoated onto 96-well plates. Standards(E.coli, K22-M93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SDF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SDF-1 amount of sample captured in plate.
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Detection Target: Stromal Cell-Derived Factor 1 (CXCL12)
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Uniprot ID: P48061)
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Reactivity: Mouse
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 62.5pg/ml-4000pg/ml
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Sensitivity: <10pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: SDF-1(stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine(C-X-C motif) ligand 12(CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice(including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.; Immunogen sequence: 350
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