Mouse Semaphorin 6A ELISA Kit

This Mouse Semaphorin 6A ELISA Kit from Innovative Research is intended for quantitative detection of mouse SEMA6A in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.

This mouse SEMA6A ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SEMA6A has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G19-T649) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEMA6A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SEMA6A amount of sample captured in plate.

• Detection Target: Semaphorin 6A
• Uniprot ID: O35464)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18; 18 C. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal. ; Immunogen sequence: 369