Mouse Regenerating Islet-Derived 3 Alpha (Reg3a) ELISA Kit

This Mouse Regenerating Islet-Derived 3 Alpha (Reg3a) ELISA Kit from Innovative Research is intended for quantitative detection of mouse REG3A in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.

This mouse REG3A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for REG3A has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: E27-Q175) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for REG3A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: Regenerating Islet-Derived 3 Alpha (Reg3a)
• Uniprot ID: O09037)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Regenerating islet-derived protein 3 alpha is a protein that in humans is encoded by the REG3A gene. It is mapped to 6 C3; 6 34.76 cM. This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known.; Immunogen sequence: 369