This Mouse Phosphatonin (FGF23) ELISA Kit from Innovative Research is intended for quantitative detection of mouse FGF23 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. This mouse FGF23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FGF23 has been precoated onto 96-well plates. Standards(E.coli, Y25-V251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF23 amount of sample captured in plate. Detection Target: Phosphatonin (FGF23) Uniprot ID: Q9EPC2) Reactivity: Mouse Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins. Range: 15.6pg/ml-1000pg/ml Sensitivity: <10pg/ml Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information:

The capture antibody is a polyclonal antibody from goat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.; Immunogen sequence: 369

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