Mouse Neurotrophin-3 (Neurotrphic Factor) ELISA Kit

This Mouse Neurotrophin-3 (Neurotrphic Factor) ELISA Kit from Innovative Research is intended for quantitative detection of mouse Neurotrophin-3 in cell culture supernates and serum. Strip well format. Reagents for up to 96 tests.

This mouse Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Neurotrophin-3 amount of sample captured in plate.

• Detection Target: Neurotrophin-3 (Neurotrphic Factor)
• Uniprot ID: P20181)
• Reactivity: Mouse
• Cross-Reactivity: There is cross-reactivates with NT-4<2.5%, and no detectable cross-reactivity with any other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <2pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.; Immunogen sequence: 290