Mouse Mannose Receptor (CD206) ELISA Kit

This Mouse Mannose Receptor (CD206) ELISA Kit from Innovative Research is intended for quantitative detection of mouse CD206/CLEC13D in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.

This mouse CD206/CLEC13D ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD206/CLEC13D has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L20-A1388) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD206/CLEC13D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD206/CLEC13D amount of sample captured in plate.

• Detection Target: Mannose Receptor (CD206)
• Uniprot ID: Q61830)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 0.78ng/ml-50ng/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). This gene is mapped to 2 A2; 2 10.46 cM. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.; Immunogen sequence: 369