This Mouse Fatty Acid Binding Protein Adipocyte (FABP4) ELISA Kit from Innovative Research is intended for quantitative detection of mouse FABP4 in cell culture supernates, serum, plasma (heparin, EDTA) and cell lysates. Strip well format. Reagents for up to 96 tests.

This mouse FABP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FABP4 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: M1-A132) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FABP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FABP4 amount of sample captured in plate.

• Detection Target: Fatty Acid Binding Protein Adipocyte (FABP4)
• Uniprot ID: P04117)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information:

The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation. ; Immunogen sequence: 333

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