Mouse Endothelial Protein C Receptor (EPCR) ELISA Kit

Mouse Endothelial Protein C Receptor (EPCR) ELISA Kit

IMSPROCRKT

Innovative Research

  • $526.00


This Mouse Endothelial Protein C Receptor (EPCR) ELISA Kit from Innovative Research is intended for quantitative detection of activated mouse EPCR in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This mouse EPCR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPCR has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L18-S214) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPCR amount of sample captured in plate.

  • Detection Target: Endothelial Protein C Receptor (EPCR)
  • Uniprot ID: Q64695)
  • Reactivity: Mouse
  • Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
  • Range: 156pg/ml-10000pg/ml
  • Sensitivity: <15pg/ml
  • Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Endothelial protein C receptor (EPCR), also known as CCCA or PROCR, is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. ; Immunogen sequence: 369

Product References
Product Disclaimer

This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.

Questions? Contact us about this product!

Get in touch with our team! Please include any relevant information (product name, etc)


We Also Recommend