Mouse Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) ELISA Kit
IMSERAP1KTThis Mouse Endoplasmic Reticulum Aminopeptidase 1 (ERAP1) ELISA Kit from Innovative Research is intended for quantitative detection of mouse ERAP1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse ERAP1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ERAP1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: M1-L930) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ERAP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ERAP1 amount of sample captured in plate.
- Detection Target: Endoplasmic Reticulum Aminopeptidase 1 (ERAP1)
- Uniprot ID: Q9EQH2)
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 312pg/ml-20000pg/ml
- Sensitivity: <50pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator, also known as endoplasmic reticulum aminopeptidase 1 (ARTS-1), is a protein which in humans is encoded by the ARTS-1 gene. This gene is mapped to 13; 13 C1. Endoplasmic reticulum amino peptidase 1 is active in the endoplasmic reticulum, which is involved in protein processing and transport. This protein is an aminopeptidase, which is an enzyme that cleaves peptides at the N-terminal into smaller fragments called amino acids.; Immunogen sequence: 369
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