Mouse Cytokine Receptor-Like Factor 2 ELISA Kit

This Mouse Cytokine Receptor-Like Factor 2 ELISA Kit from Innovative Research is intended for Quantitative Detection of mouse CRLF2 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This mouse CRLF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for CRLF2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A20 - L233) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRLF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CRLF2 amount of sample captured in plate.

• Detection Target: Cytokine Receptor-Like Factor 2
• Uniprot ID: Q8CII9)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 125pg/ml-8000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin(TSLP). Together with the interleukin 7 receptor(IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.; Immunogen sequence: 369