Mouse Cellular Repressor of E1A-Stimulated Genes 1 (CREG1) ELISA Kit

This Mouse Cellular Repressor of E1A-Stimulated Genes 1 (CREG1) ELISA Kit from Innovative Research is intended for quantitative detection of mouse CREG1 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.

This mouse CREG1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CREG1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: R32-Q220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CREG1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CREG1 amount of sample captured in plate.

• Detection Target: Cellular Repressor of E1A-Stimulated Genes 1 (CREG1)
• Uniprot ID: O88668)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Protein CREG1 (Cellular Repressor of E1A-stimulated Genes 1) is a protein that in humans is encoded by the CREG1 gene. It is mapped to 1; 1 H2.3. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.; Immunogen sequence: 369