Mouse Carboxypeptidase E ELISA Kit

This Mouse Carboxypeptidase E ELISA Kit from Innovative Research is intended for quantitative detection of mouse CPE in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This mouse CPE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CPE has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L43-F476) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CPE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: Carboxypeptidase E
• Uniprot ID: Q00493)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: CPE encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. It is mapped to 8 B3.1; 8 32.3 cM. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin.; Immunogen sequence: 369