Mouse Carbonic Anhydrase 12 ELISA Kit

This Mouse Carbonic Anhydrase 12 ELISA Kit from Innovative Research is intended for quantitative detection of mouse CA12 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.

This mouse SEZ6L ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SEZ6L has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A25-S301) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEZ6L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SEZ6L amount of sample captured in plate.

• Detection Target: Carbonic Anhydrase 12
• Uniprot ID: Q8CI85)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 9; 9 C. This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.; Immunogen sequence: 369