Mouse Bone Morphogenetic Protein 6 ELISA Kit

This Mouse Bone Morphogenetic Protein 6 ELISA Kit from Innovative Research is intended for Quantitative Detection of mouse BMP-6 in bone tissue, cell culture supernates and serum. Strip well format. Reagents for up to 96 tests.

This mouse BMP-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-6 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: S372-H510) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-6 amount of sample captured in plate.

• Detection Target: Bone Morphogenetic Protein 6
• Uniprot ID: P20722)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Bone morphogenetic protein 6 is a protein that in humans is encoded by the BMP6 gene. It is mapped to 6p24-p23. The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.; Immunogen sequence: 369