
Mouse Amphiregulin-Associated Protein (Midkine) ELISA Kit
IMSMDKKTThis Mouse Amphiregulin-Associated Protein (Midkine) ELISA Kit from Innovative Research is intended for quantitative detection of mouse Midkine in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This mouse Midkine ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Midkine has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: K23-D140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Midkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Midkine amount of sample captured in plate.
- Detection Target: Amphiregulin-Associated Protein (Midkine)
- Uniprot ID: P12025)
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 15.6pg/ml-1000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Midkine (MK) is a member of a family of neurotrophic and developmentally-regulated heparin-binding molecules that currently consists of midkine, pleiotrophin/PTN and avian RI-HB. This gene is mapped to 2 E1; 2 50.63 cM. This gene encodes a secreted growth factor that belongs to the pleiotrophin/midkine heparin-binding protein family and functions in a variety of biological processes. The encoded cytokine promotes the growth, differentiation, survival and migration of several target cells including leucocytes involved in inflammation. This protein plays a role in the formation of scar tissue and intraperitoneal adhesions, and promotes neurite outgrowth and neuron survival. The protein encoded by this gene is associated with obesity and inhibition of insulin signaling in fat cells. A pseudogene of this gene is present on chromosome 11. Alternative splicing results in multiple transcript variants.; Immunogen sequence: 369
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