Monkey Macrophage Migration Inhibitory Factor High-Sensitivity ELISA Kit

This Monkey Macrophage Migration Inhibitory Factor High-Sensitivity ELISA Kit from Innovative Research is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of monkey primate MIF. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.

This Monkey MIF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIF has been precoated onto 96-well plates. Standards(E.coli, P2-A115) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey MIF amount of sample captured in plate.

• Detection Target: Macrophage Migration Inhibitory Factor
• Uniprot ID: I4AY87)
• Reactivity: Monkey
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10,000pg/ml
• Sensitivity: <20pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: Macrophage migration inhibitory factor(MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to monkey primate chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.; Immunogen sequence: 400