Monkey Brain-Derived Neurotrophic Factor High-Sensitivity ELISA KitIMNBDNFKTH
This Monkey Brain-Derived Neurotrophic Factor High-Sensitivity ELISA Kit from Innovative Research is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of monkey primate BDNF. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.
This Monkey BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BDNF has been precoated onto 96-well plates. Standards(sf21, H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey BDNF amount of sample captured in plate.
- Detection Target: Brain-Derived Neurotrophic Factor
- Uniprot ID: P23560)
- Reactivity: Monkey
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 31.2pg/ml-2000pg/ml
- Sensitivity: <15pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult monkey primate brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding monkey primate BDNF to chromosome 11, band p13.; Immunogen sequence: 270
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.
Get in touch with our team! Please include any relevant information (product name, etc)